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	Provedor de dados BJID (3) Autor Pereira-Chioccola,Vera Lucia (3) Vidal,Jose Ernesto (2) Barbosa,Amanda Pires (1) Barbosa,Deusenia Machado Ulisses (1) Brighente,Kate Bastos Santos (1) Camilo,Lilian Muniz (1) Catelan,Marcia Wakai (1) Cavallini,Danilo (1) De Mattos,Luiz Carlos (1) Dias,Cristiane Moraes (1) Ferreira,Marina Neves (1) Frederico,Fábio Batista (1) Gava,Ricardo (1) Gonçalves,Fausto da Silva (1) Hipólito,Daise Damaris Carnietto de (1) Martins,Marilena dos Anjos (1) Matos,Terezinha Aparecida de (1) Mattos,Cinara Cássia Brandão de (1) Meira-Strejevitch,Cristina da Silva (1) Mesquita,Rafael Tonini (1) Mais... Palavra-chave AIDS (1) Acquired immunodeficiency syndrome (1) Cerebral toxoplasmosis (1) Cerebrospinal fluid (1) Cryptococcal meningitis (1) Diagnosis (1) Molecular diagnosis (1) PCR markers (1) Polymerase chain reaction (1) Real-time polymerase chain reaction (1) Symptomatic toxoplasmosis (1) Toxoplasma gondii (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2017 (1) 2015 (1) 2010 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Molecular diagnosis of cerebral toxoplasmosis: comparing markers that determine Toxoplasma gondii by PCR in peripheral blood from HIV-infected patients BJID Mesquita,Rafael Tonini; Vidal,José Ernesto; Pereira-Chioccola,Vera Lucia. As cerebral toxoplasmosis is the most common cerebral focal lesion in AIDS patients, this study evaluated three PCR markers for diagnosis, since some limitations remain present, such as low parasite levels in some clinical samples. The molecular markers were B22-B23 and Tg1-Tg2 (based on the B1 gene) and Tox4-Tox5 (non-coding fragment, repeated 200-300-fold). DNA samples from 102 AIDS patients with previously known diagnosis were analyzed. The cerebral toxoplasmosis group was constituted of DNA extracted from the blood of 66 AIDS patients, which was collected before or until the third day of the therapy for toxoplasmosis. DNA from the blood of 36 AIDS patients with other neurologic opportunistic infections was used as control group. Sensitivities of... Tipo: Info:eu-repo/semantics/article Palavras-chave: AIDS; Cerebral toxoplasmosis; Molecular diagnosis; PCR markers. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400006 Molecular diagnosis of cryptococcal meningitis in cerebrospinal fluid: comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex BJID Martins,Marilena dos Anjos; Brighente,Kate Bastos Santos; Matos,Terezinha Aparecida de; Vidal,Jose Ernesto; Hipólito,Daise Damaris Carnietto de; Pereira-Chioccola,Vera Lucia. Aim:This study evaluated the use of polymerase chain reaction for cryptococcal meningitis diagnosis in clinical samples.Materials and methods:The sensitivity and specificity of the methodology were evaluated using eight Cryptococcus neoformans/C. gattiispecies complex reference strains and 165 cere- brospinal fluid samples from patients with neurological diseases divided into two groups: 96 patients with cryptococcal meningitis and AIDS; and 69 patients with other neurological opportunistic diseases (CRL/AIDS). Two primer sets were tested (CN4-CN5 and the multiplex CNa70S-CNa70A/CNb49S-CNb-49A that amplify a specific product for C. neoformansand another for C. gattii).Results:CN4-CN5 primer set was positive in all Cryptococcusstandard strains and in 94.8% in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptococcal meningitis; Acquired immunodeficiency syndrome; Polymerase chain reaction; Cerebrospinal fluid. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000100062 Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil BJID Camilo,Lilian Muniz; Pereira-Chioccola,Vera Lucia; Gava,Ricardo; Meira-Strejevitch,Cristina da Silva; Vidal,Jose Ernesto; Mattos,Cinara Cássia Brandão de; Frederico,Fábio Batista; De Mattos,Luiz Carlos; Spegiorin,Lígia Cosentino Junqueira Franco; Murata,Fernando Henrique Antunes; Ferreira,Marina Neves; Barbosa,Deusenia Machado Ulisses; Gonçalves,Fausto da Silva; Dias,Cristiane Moraes; Catelan,Marcia Wakai; Siqueira,Rubens Camargo; Previato,Mariana; Barbosa,Amanda Pires; Cavallini,Danilo. ABSTRACT Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA. The methodology was assayed in 807 clinical samples with known clinical diagnosis, ELISA, and conventional PCR results in a 9-year period. All samples were from patients with clinical suspicion of several features of toxoplasmosis. According to the minimum detection limit curve (in CT), REP-529 had greater sensitivity to detect T. gondii DNA than B1. Both primer sets were retrospectively evaluated using 515... Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasma gondii; Symptomatic toxoplasmosis; Diagnosis; Real-time polymerase chain reaction. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600638 Registros recuperados: 3 Primeira ... 1 ... Última

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